An Improved and Reliable Method for the Induction of Colitis in Rats Using 2,4,6-Trinitrobenzene Sulfonic Acid

An Improved and Reliable Method for the Induction of Colitis in Rats Using 2,4,6-Trinitrobenzene Sulfonic Acid

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We performed this study to develop a reliable method for inducing colitis in rats using 2,4,6-trinitrobenzene sulfonic acid (TNBS) to reduce the variation in ulcer size.A pair of ring forceps was used to clamp the colon and 0.1 M TNBS in ethanol was injected into the Autres luminal side of the clamped portion.This method resulted in a small coefficient of variation of the ulcer index.A significant linearity was observed by plotting ulcer size against days after ulcer induction in #7 MEDIUM BLOND both logarithm scales.

These findings show that this technique is simple and reliable and that ulcers heal linearly.Keywords:: inflammatory bowel disease, colitis model, 2,4,6-trinitrobenzene sulfonic acid (TNBS).